All ties to be played week commencing 1 December 2025
Northern Section
Lincoln City v Huddersfield Town
Doncaster Rovers v Chesterfield
Tranmere Rovers v Fleetwood Town
Salford City v Rotherham United
Port Vale v Barnsley
Harrogate Town v Blackpool
Bolton Wanderers v Bradford City
Crewe Alexandra v Stockport County
Southern Section
Bristol Rovers v Cambridge United
Swindon Town v Peterborough United
Cardiff City v AFC Wimbledon
Northampton Town v Wycombe Wanderers
Colchester United v West Ham United U21
Luton Town v Exeter City
Stevenage v Walsall
Leyton Orient v Plymouth Argyle